West Mexico Berries Modulate α-Amylase, α-Glucosidase and Pancreatic Lipase Using In Vitro and In Silico Approaches.

The objective was to evaluate the antioxidant and biological potential of eight freeze-dried berry varieties of southern Jalisco using in silico and in vitro approaches. Fourteen tentative phenolic compounds were identified in berries by ESI-QToF, including anthocyanins, phenolic acids, flavanols and flavonols. In silico assays of phytochemicals in the berry inhibiting enzymes related to obesity and diabetes showed predicted binding energy interactions (ranging from -5.4 to -9.3 kcal/mol). Among the cultivars, antioxidant potential for DPPH IC 50 ranged from 1.27 to 3.40 mg/mL, ABTS IC 50 from 2.26 to 7.32 mg/mL and nitric oxide (NO) inhibition IC 50 from 4.26 to 11.07 mg/mL. The potential to inhibit α-amylase IC 50 ranged from 4.02 to 7.66 mg/mL, α-glucosidase IC 50 from 0.27 to 4.09 mg/mL, lipase IC 50 from 1.30 to 4.82 mg/mL and DPP-IV IC 50 from 1.36 to 3.31 mg/mL. Blackberry cultivars from the southern Jalisco region showed outstanding biological potential compared to other evaluated berries and could be used in the formulation of functional foods in the prevention of noncommunicable diseases.