A SERS/fluorescence dual-mode immuno-nanoprobe for investigating two anti-diabetic drugs on EGFR expressions.
Yuqi ChengLili CongXiaozhang QuJunyi ZhaoJiamin ChenPing LiWei ShiWeiqing XuShu-Ping XuPublished in: Mikrochimica acta (2023)
A surface-enhanced Raman scattering (SERS)/fluorescence dual-mode nanoprobe was proposed to assess anti-diabetic drug actions from the expression level of the epidermal growth factor receptor (EGFR), which is a significant biomarker of breast cancers. The nanoprobe has a raspberry shape, prepared by coating a dye-doped silica nanosphere with a mass of SERS tags, which gives high gains in fluorescence imaging and SERS measurement. The in situ detection of EGFR on the cell membrane surfaces after drug actions was achieved by using this nanoprobe, and the detection results agree with the enzyme-linked immunosorbent assay (ELISA) kit. Our study suggests that rosiglitazone hydrochloride (RH) may be a potential drug for diabetic patients with breast cancer, while the anti-cancer effect of metformin hydrochloride (MH) is debatable since MH slightly promotes the EGFR expression of MCF-7 cells in this study. This sensing platform endows more feasibility for highly sensitive and accurate feedback of pesticide effects at the membrane protein level.
Keyphrases
- epidermal growth factor receptor
- tyrosine kinase
- small cell lung cancer
- label free
- gold nanoparticles
- advanced non small cell lung cancer
- sensitive detection
- fluorescence imaging
- type diabetes
- living cells
- poor prognosis
- raman spectroscopy
- high throughput
- wound healing
- risk assessment
- single molecule
- induced apoptosis
- emergency department
- drug induced
- loop mediated isothermal amplification
- high resolution
- mass spectrometry
- binding protein
- signaling pathway
- energy transfer
- climate change
- human health
- molecularly imprinted
- cell cycle arrest
- metal organic framework