Neonatal Streptococcus pneumoniae Pneumonia Induces an Aberrant Airway Smooth Muscle Phenotype and AHR in Mice Model.
Xin PengYi WuXiao KongYunxiu ChenYonglu TianQinyuan LiXiaoyin TianGuangli ZhangLuo RenZhengxiu LuoPublished in: BioMed research international (2019)
Our previous study showed that neonatal S. pneumoniae infection aggravated airway inflammation and airway hyperresponsiveness (AHR) in an OVA-induced allergic asthma model. As airway smooth muscle (ASM) plays a pivotal role in AHR development, we aim to investigate the effects of neonatal S. pneumoniae pneumonia on ASM structure and AHR development. Non-lethal neonatal pneumonia was established by intranasally infecting 1-week-old BALB/C mice with the S. pneumoniae strain D39. Five weeks after infection, the lungs were collected to assess the levels of α-SMA and the contractile proteins of ASM. Our results indicate that neonatal S. pneumoniae pneumonia significantly increased adulthood lung α-SMA and SMMHC proteins production and aggravated airway inflammatory cells infiltration and cytokines release. In addition, the neonatal S. pneumoniae pneumonia group had significantly higher Penh values compared to the uninfected controls. These data suggest that neonatal S. pneumoniae pneumonia promoted an aberrant ASM phenotype and AHR development in mice model.
Keyphrases
- smooth muscle
- community acquired pneumonia
- high fat diet induced
- respiratory tract
- respiratory failure
- type diabetes
- chronic obstructive pulmonary disease
- skeletal muscle
- randomized controlled trial
- hiv infected
- machine learning
- clinical trial
- intensive care unit
- induced apoptosis
- metabolic syndrome
- wild type
- endothelial cells
- high glucose
- study protocol
- endoplasmic reticulum stress