Andrographolide Inhibition of Th17-Regulated Cytokines and JAK1/STAT3 Signaling in OVA-Stimulated Asthma in Mice.
Qian YuYaJie ShiChang ShuXuChun DingShiPing ZhuZhouPing ShenYaFang LouPublished in: Evidence-based complementary and alternative medicine : eCAM (2021)
Asthma has long been considered a disease of airway inflammation. The excessive or prolonged production of inflammatory mediators can result in airway remodeling and severe clinical syndromes such as dyspnea or even apnea. Therefore, pharmaceutical intervention is required to restrain the excessive release of such inflammatory mediators in control of asthma. Novel therapeutics and mechanistic insight are sought for the management of this chronic inflammatory disease. Andrographolide (AG) is a type of diterpenoid ester compound and is reported to demonstrate multiple properties such as antioxidation and anti-inflammation. However, the anti-inflammatory capacity of AG by regulating immunologic function in airway of asthma has not been fully studied to date. Therefore, this study investigates whether AG is capable of suppressing the inflammatory response of asthma in OVA-stimulated mice and the mechanism by which this is achieved. Animals were randomly divided into 4 groups: control group, OVA model group, OVA + AG (0.1 mg/ml) group, and OVA + dimethylsulfoxide (DMSO) group. The serum, BALF, and lung tissue of the mice were collected separately for the administration of ELISA, rt-PCR, western blot and pathological section and staining. We found that AG attenuated the OVA-induced production of IL-6, IL-17A, IL-17F, and RORγt; inhibited the OVA-mediated phosphorylation of JAK 1 and STAT3; and alleviated airway remodeling and the neutrophil infiltration of lung tissue. We conclude that AG inhibits the inflammatory response of asthma in OVA-stimulated mice by blocking the activation of Th17-regulated cytokines and the JAK1/STAT3 signaling pathway.
Keyphrases
- chronic obstructive pulmonary disease
- lung function
- inflammatory response
- quantum dots
- allergic rhinitis
- oxidative stress
- signaling pathway
- high fat diet induced
- highly efficient
- randomized controlled trial
- anti inflammatory
- transcription factor
- cystic fibrosis
- small molecule
- lipopolysaccharide induced
- air pollution
- insulin resistance
- metabolic syndrome
- lps induced
- drug induced
- cell proliferation
- type diabetes
- epithelial mesenchymal transition
- wild type
- body mass index