Proteomic analysis of human synovial fluid reveals potential diagnostic biomarkers for ankylosing spondylitis.

Ji-Hyun LeeJae Hun JungJeesoo KimWon-Ki BaekJinseol RheeTae-Hwan KimSang-Hyon KimKwang-Pyo Kim Chang-Nam Son Jong-Seo Kim

Published in: Clinical proteomics (2020)

We performed quantitative comparative proteomic analysis using by LC-MS/MS of the SF from four disease states: RA, gout, and OA. This systematic comparison revealed novel differentially expressed proteins in AS SF, as well as two previously reported candidate biomarkers. We further verified the expression of MMP3, C9 and CFHR5 by western blot. These proteins may serve as diagnostic or prognostic biomarkers in patients with AS, and may thus improve the clinical outcomes of this serious disease.

Keyphrases

ankylosing spondylitis
rheumatoid arthritis
disease activity
endothelial cells
poor prognosis
high resolution
uric acid
south africa
binding protein
induced pluripotent stem cells
mass spectrometry
long non coding rna
idiopathic pulmonary fibrosis