RNA interference-based strategies to control Botrytis cinerea infection in cultivated strawberry.
Capriotti LucaMolesini BarbaraPandolfini TizianaJin HailingBaraldi ElenaCecchin MichelaMezzetti BrunoSabbadini SilviaPublished in: Plant cell reports (2024)
of naked BcDCL1/2 dsRNA, sprayed on plants of Fragaria x ananassa cultivar Romina in the greenhouse, displayed significant reduction of susceptibility, compared to the negative controls, but to a lesser extent than the chemical fungicide. Three independent lines of Romina cultivar were confirmed for their stable expression of the hairpin gene construct that targets the Bc-DCL1 and 2 sequences (hp-Bc-DCL1/2), and for the production of hp construct-derived siRNAs, by qRT-PCR and Northern blot analyses. In vitro and in vivo detached leaves, and fruits from the hp-Bc-DCL1/2 lines showed significantly enhanced tolerance to this fungal pathogen compared to the control. This decreased susceptibility was correlated to the reduced fungal biomass and the downregulation of the Bc-DCL1 and 2 genes in B. cinerea. These results confirm the potential of both RNAi-based products and plants for protecting the cultivated strawberry from B. cinerea infection, reducing the impact of chemical pesticides on the environment and the health of consumers.
Keyphrases
- genome wide
- healthcare
- public health
- poor prognosis
- risk assessment
- genome wide identification
- mental health
- human health
- signaling pathway
- copy number
- gene expression
- dna methylation
- health information
- climate change
- genome wide analysis
- gas chromatography
- anaerobic digestion
- social media
- bioinformatics analysis
- solid phase extraction