Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system.

David Ruano-GallegoSofía FraileCarlos GutierrezLuis Ángel Fernández

Published in: Microbial cell factories (2019)

Our data demonstrate the Hly secretion system of E. coli can be used as an expression platform for screening and purification of Nb binders from VHH repertories.

Keyphrases

escherichia coli
poor prognosis
electronic health record
high throughput
big data
recombinant human
machine learning
artificial intelligence