Purshia plicata Triggers and Regulates Proteins Related to Apoptosis in HeLa Cancer Cells.
Patricia Álvarez-OrtizAscacio-Valdés JuanIleana Vera-ReyesCecilia Esparza-GonzálezRaul Rodríguez-HerreraMauricio Andres Salinas-SantanderMayela Del Ángel-MartínezMorlett-Chávez JesúsPublished in: Plants (Basel, Switzerland) (2021)
Cervical cancer represents a public health problem, develops resistance to traditional therapies and cost-of-treatment is high. These disadvantages have led to the search for alternative bioactive-compound-based therapies. Said bioactive compounds include phenolic compounds, flavonoids, and tannins. The present study aimed to evaluate the therapeutic effect of a P. plicata extract on the HeLa cell line. Viability and apoptosis assays were run on the two cell lines treated with the extract. The peptides, up- and down-expressed in both cell lines, were identified by PDQuest analysis software and high-performance liquid chromatography/mass spectrometry/mass spectrometry (HPLC/MS/MS). Our results show that a 500 mg/L treatment deregulated cell viability, with different apoptotic morphologies observed which are associated with the presence of bio-compounds, which up- and down-regulated the peptides. In conclusion, P. plicata regulates proteins associated with apoptosis in HeLa cancer cells.
Keyphrases
- high performance liquid chromatography
- mass spectrometry
- cell cycle arrest
- cell death
- oxidative stress
- ms ms
- public health
- tandem mass spectrometry
- simultaneous determination
- liquid chromatography
- endoplasmic reticulum stress
- solid phase extraction
- liquid chromatography tandem mass spectrometry
- high resolution
- combination therapy
- high throughput
- signaling pathway
- drug induced
- newly diagnosed
- replacement therapy
- high resolution mass spectrometry