Dysregulation of extracellular vesicle protein cargo in female ME/CFS cases and sedentary controls in response to maximal exercise.
Ludovic GiloteauxKatherine A GlassArnaud GermainSheng ZhangMaureen R HansonPublished in: bioRxiv : the preprint server for biology (2023)
In healthy individuals, physical exercise improves cardiovascular health and muscle strength, alleviates fatigue, and reduces risk of chronic diseases. Although exercise is suggested as a lifestyle intervention to manage various chronic illnesses, it negatively affects people with myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), who suffer from exercise intolerance. We hypothesized that altered extracellular vesicle (EV) signaling in ME/CFS patients after an exercise challenge may contribute to their prolonged and exacerbated negative response to exertion (post-exertional malaise). EVs were isolated by size exclusion chromatography from the plasma of 18 female ME/CFS patients and 17 age- and BMI-matched female sedentary controls at three time points: before, 15 minutes, and 24 hours after a maximal cardiopulmonary exercise test. EVs were characterized using nanoparticle tracking analysis and their protein cargo was quantified using Tandem Mass Tag-based (TMT) proteomics. The results show that exercise affects the EV proteome in ME/CFS patients differently than in healthy individuals and that changes in EV proteins after exercise are strongly correlated with symptom severity in ME/CFS. Differentially abundant proteins in ME/CFS patients vs. controls were involved in many pathways and systems, including coagulation processes, muscle contraction (both smooth and skeletal muscle), cytoskeletal proteins, the immune system, and brain signaling.
Keyphrases
- end stage renal disease
- physical activity
- high intensity
- ejection fraction
- skeletal muscle
- chronic kidney disease
- newly diagnosed
- resistance training
- peritoneal dialysis
- randomized controlled trial
- prognostic factors
- mass spectrometry
- multiple sclerosis
- type diabetes
- adipose tissue
- patient reported outcomes
- body mass index
- insulin resistance
- resting state
- brain injury
- weight gain
- high resolution
- smooth muscle
- protein protein
- heat stress
- functional connectivity