Enhancing the biochemical growth of Haematococcus pluvialis by mitigation of broad-spectrum light stress in wastewater cultures.

In this study, the microalgae Haematococcus pluvialis were cultivated in wastewater inoculated into low-density polypropylene plastic air pillows (LDPE-PAPs) under a light stress. The cells were irradiated to different light stresses using white LED lights (WLs) as the control, and broad-spectrum lights (BLs) as a test for the period of 32 days. It was observed that the inoculum (70 × 10 2 mL -1 cells) of H. pluvialis algal cells increased almost 30 and 40 times in WL and BL, respectively, at day 32 coherent to its biomass productivity. Higher lipid concentration of up to 36.85 μg mL -1 was observed in BL irradiated cells compared to 13.215 μg L -1 dry weight of biomass in WL. The chlorophyll 'a' content was 2.6 times greater in BL (3.46 μg mL -1 ) compared to that in WL (1.32 μg mL -1 ) with total carotenoids being about 1.5 times greater in BL compared to WL on day 32. The yield of red pigment 'Astaxanthin' was about 27% greater in BL than in WL. The presence, of different carotenoids including astaxanthin was also confirmed by HPLC, whereas fatty acid methyl esters (FAMEs) were confirmed by GC-MS. This study further confirmed that wastewater alongwith with light stress is suitable for the biochemical growth of H. pluvialis with good biomass yield as well as carotenoid accumulation. Additionally there was 46% reduction in chemical oxygen demand (COD) in a far more efficient manner when cultured in recycled LDPE-PAP. Such type of cultivation of H. pluvialis made the overall process economical and suitable for upscaling to produce value-added products such as lipids, pigments, biomass, and biofuel for commercial applications.