Evaluating the performance of OCT in assessing static and potential dynamic properties of the retinal ganglion cells and nerve fiber bundles in the living mouse eye.
Pengfei ZhangOlga VafaevaChristian DolfYanhong MaGuozhen WangJessicca ChoHenry Ho-Lung ChanNicholas Marsh-ArmstrongRobert J ZawadzkiPublished in: Biomedical optics express (2023)
Glaucoma is a group of eye diseases characterized by the thinning of the retinal nerve fiber layer (RNFL), which is primarily caused by the progressive death of retinal ganglion cells (RGCs). Precise monitoring of these changes at a cellular resolution in living eyes is significant for glaucoma research. In this study, we aimed to assess the effectiveness of temporal speckle averaging optical coherence tomography (TSA-OCT) and dynamic OCT (dOCT) in examining the static and potential dynamic properties of RGCs and RNFL in living mouse eyes. We evaluated parameters such as RNFL thickness and possible dynamics, as well as compared the ganglion cell layer (GCL) soma density obtained from in vivo OCT, fluorescence scanning laser ophthalmoscopy (SLO), and ex vivo histology.
Keyphrases
- optical coherence tomography
- optic nerve
- diabetic retinopathy
- induced apoptosis
- cell cycle arrest
- randomized controlled trial
- single cell
- single molecule
- endoplasmic reticulum stress
- multiple sclerosis
- systematic review
- cell death
- spinal cord
- peripheral nerve
- mesenchymal stem cells
- bone marrow
- pi k akt
- neuropathic pain
- energy transfer