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Exosome-like vesicles in uterine aspirates: a comparison of ultracentrifugation-based isolation protocols.

Irene CampoyLucia LanauTatiana AltadillTamara SequeirosSilvia CabreraMontserrat Cubo-AbertAssumpción Pérez-BenaventeAngel GarciaSalvador BorrósAnna SantamariaJordi PonceXavier Matias-GuiuJaume ReventósAntonio Gil-MorenoMarina RigauEva Colas
Published in: Journal of translational medicine (2016)
We confirmed the existence of exosome-like vesicles in the fluid fraction of uterine aspirates. They were successfully isolated by differential centrifugation giving sufficient proteomic and transcriptomic material for further analyses. The Standard protocol was the best performing procedure since the other two tested protocols did not ameliorate neither yield nor purity of exosome-like vesicles. This study contributes to establishing the basis for future comparative studies to foster the field of biomarker research in gynecology.
Keyphrases
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