Maresin 1 inhibits TNF-alpha-induced lipolysis and autophagy in 3T3-L1 adipocytes.
Laura M LaiglesiaSilvia Lorente-CebriánMiguel López-YoldiRaquel LanasNeira SáinzJose Alfredo MartínezMaría Jesús Moreno-AliagaPublished in: Journal of cellular physiology (2017)
Obesity is associated with high levels of pro-inflammatory cytokines such as tumor necrosis factor-alpha (TNF-α), which promotes inflammation in adipose tissue. The omega-3 PUFAs, and their derived lipid mediators, such as Maresin 1 (MaR1) have anti-inflammatory effects on adipose tissue. This study aimed to analyze if MaR1 may counteract alterations induced by TNF-α on lipolysis and autophagy in mature 3T3-L1 adipocytes. Our data revealed that MaR1 (1-100 nM) inhibited the TNF-α-induced glycerol release after 48 hr, which may be related to MaR1 ability of preventing the decrease in lipid droplet-coating protein perilipin and G0/G1 Switch 2 protein expression. MaR1 also reversed the decrease in total hormone sensitive lipase (total HSL), and the ratio of phosphoHSL at Ser-565/total HSL, while preventing the increased ratio of phosphoHSL at Ser-660/total HSL and phosphorylation of extracellular signal-regulated kinase 1/2 induced by TNF-α. Moreover, MaR1 counteracted the cytokine-induced decrease of p62 protein, a key autophagy indicator, and also prevented the induction of LC3II/LC3I, an important autophagosome formation marker. Current data suggest that MaR1 may ameliorate TNF-α-induced alterations on lipolysis and autophagy in adipocytes. This may also contribute to the beneficial actions of MaR1 on adipose tissue and insulin sensitivity in obesity.
Keyphrases
- adipose tissue
- insulin resistance
- rheumatoid arthritis
- oxidative stress
- diabetic rats
- high fat diet
- high glucose
- cell death
- endoplasmic reticulum stress
- anti inflammatory
- type diabetes
- high fat diet induced
- metabolic syndrome
- drug induced
- weight loss
- electronic health record
- high throughput
- fatty acid
- body mass index
- tyrosine kinase
- small molecule
- data analysis
- protein protein
- weight gain
- machine learning
- deep learning
- physical activity