Rapid Androgen-Responsive Proteome Is Involved in Prostate Cancer Progression.
Jong-Kwang KimJae-Hun JungDong Hoon ShinHye Jin YouSeho ChaBo-Seul SongJae-Young JoungWeon Seo ParkKwang-Pyo KimJae-Kyung MyungPublished in: Biomedicines (2021)
Androgen exerts its functions by binding with an androgen receptor (AR). It can activate many signaling pathways that are important to the progression of castration-resistant prostate cancer (CRPC). Here, we characterized the rapid proteomic changes seen at 5, 15, 30, and 60 min after the androgen treatment of VCaP cells via the tandem mass tag (TMT) labeling strategy. A total of 5529 proteins were successfully identified and quantified. Dynamic time profiling of protein expression patterns allowed us to identify five protein clusters involved in various stages of androgen-initiated signal transmission and processing. More details of protein functions and localization patterns, and our elucidation of an AR-interacting protein network, were obtained. Finally, we validated the expression level of AR-regulated proteins known to be significantly regulated in CRPC patients using the mouse xenograft model and patient samples. Our work offers a systematic analysis of the rapid proteomic changes induced by androgen and provides a global view of the molecular mechanisms underlying CRPC progression.
Keyphrases
- prostate cancer
- binding protein
- protein protein
- induced apoptosis
- end stage renal disease
- transcription factor
- newly diagnosed
- signaling pathway
- loop mediated isothermal amplification
- chronic kidney disease
- amino acid
- ejection fraction
- poor prognosis
- case report
- oxidative stress
- prognostic factors
- small molecule
- epithelial mesenchymal transition
- cell cycle arrest
- endoplasmic reticulum stress
- cell death
- single molecule
- long non coding rna
- pi k akt
- patient reported
- smoking cessation
- dna binding