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Detection and genetic characterization of feline retroviruses in domestic cats with different clinical signs and hematological alterations.

Gabriel Eduardo Acevedo-JiménezRosa Elena Sarmiento-SilvaRogelio Alejandro Alonso-MoralesRodolfo Córdova-PonceHugo Ramírez Álvarez
Published in: Archives of virology (2022)
Feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV) are globally distributed retroviruses that infect domestic cats and cause various syndromes that can lead to death. The aim of this study was to detect and genotype feline retroviruses in Mexican domestic cats. We used PCR assays to identify proviral DNA and viral RNA in 50 domestic cats with different clinical signs and hematological alterations. Endogenous FeLV (enFeLV) was identified in the genomic DNA of all cats in the study, and we detected transcripts of the LTR region of enFeLV in 48 individuals. Exogenous FeLV (exFeLV) was found in 13 cats. Furthermore, we detected FIV proviral DNA in 10 cats. The enFeLV sequences were shown to be the most variable, while the exFeLV sequences were highly conserved and related to previously reported subgroup A sequences. Sequencing of the FIV gag gene revealed the presence of subtype B in the infected cats.
Keyphrases
  • circulating tumor
  • single molecule
  • cell free
  • randomized controlled trial
  • single cell
  • gene expression
  • nucleic acid
  • high throughput
  • genome wide
  • open label
  • genetic diversity
  • phase iii
  • double blind