In vitro induction of NETosis: Comprehensive live imaging comparison and systematic review.
Tamara HoppenbrouwersAnouchska S A AutarAndi R SultanTsion E AbrahamWiggert A van CappellenAdriaan B HoutsmullerWillem J B van WamelHeleen M M van BeusekomJohan W van NeckMoniek P M de MaatPublished in: PloS one (2017)
Our systematic review confirms that there is much variation in study design and results of NETosis induction. Our experimental results confirm that under standardized conditions, PMA, living bacteria and Ionomycin all strongly induce NETosis, but real-time confocal imaging reveal different courses of events.