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CRISPR-Cas12a-based genome editing and transcriptional repression for biotin synthesis in Pseudomonas mutabilis.

Jiarun ZhaoSiqi ZuoLei HuangJiazhang LianZhinan Xu
Published in: Journal of applied microbiology (2023)
This CRISPR-Cas12a system is an efficient genome editing and regulation tool to facilitate the construction of P. mutabilis cell factories.
Keyphrases
  • genome editing
  • crispr cas
  • single cell
  • gene expression
  • transcription factor
  • biofilm formation
  • stem cells
  • oxidative stress
  • escherichia coli
  • pseudomonas aeruginosa
  • bone marrow
  • staphylococcus aureus
  • cystic fibrosis