Fluorescent Turn-On Anthracene-Based Aluminum(III) Sensor for a Therapeutic Study in Alzheimer's Disease Model of Drosophila .
Gautam KumarAnanya SrivastavaPrabhat KumarSaripella SrikrishnaVinod P SinghPublished in: ACS chemical neuroscience (2023)
A new anthracene-based probe ( E )- N '-(1-(anthracen-9-yl)ethylidene)-2-hydroxybenzohydrazide ( AHB ) has been efficiently synthesized and characterized by various spectroscopic methods. It exhibits extremely selective and sensitive fluorometric sensing of Al 3+ ions with a large enhancement in the fluorescent intensity due to the restricted photoinduced electron transfer (PET) mechanism with a chelation-enhanced fluorescence (CHEF) effect. The AHB -Al 3+ complex shows a remarkably low limit of detection at 0.498 nM. The binding mechanism has been proposed based on Job's plot, 1 H NMR titration, Fourier transform infrared (FT-IR), high-resolution mass spectrometry (HRMS), and density functional theory (DFT) studies. The chemosensor is reusable and reversible in the presence of ctDNA. The practical usability of the fluorosensor has been established by a test strip kit. Further, the therapeutic potential of AHB against Al 3+ ion-induced tau protein toxicity has been tested in the eye of Alzheimer's disease (AD) model of Drosophila via metal chelation therapy. AHB shows great therapeutic potential with 53.3% rescue in the eye phenotype. The in vivo interaction study of AHB with Al 3+ in the gut tissue of Drosophila confirms its sensing efficiency in the biological environment. A detailed comparison table included evaluates the effectiveness of AHB .
Keyphrases
- density functional theory
- high resolution mass spectrometry
- quantum dots
- living cells
- electron transfer
- randomized controlled trial
- liquid chromatography
- magnetic resonance
- systematic review
- high glucose
- healthcare
- computed tomography
- fluorescent probe
- electronic health record
- high intensity
- endothelial cells
- positron emission tomography
- pet imaging
- diabetic rats
- simultaneous determination
- molecular dynamics simulations
- tandem mass spectrometry