Overexpression of a soybean Globin (GmGlb1-1) gene reduces plant susceptibility to Meloidogyne incognita.
Marcos Fernando BassoIsabela Tristan Lourenço-TessuttiClidia Eduarda Moreira-PintoReneida Aparecida Godinho MendesBruno Paes-de-MeloMaysa Rosa das NevesAmanda Ferreira MacedoViviane FigueiredoAdriana GrandisLeonardo Lima Pepino MacedoFabrício Barbosa Monteiro ArraesMarcos Mota do Carmo CostaRoberto Coiti TogawaAlex Enrich-PrastFrancismar Corrêa Marcelino-GuimaraesAna Cristina Meneses Mendes GomesMaria Cristina Mattar SilvaEny Iochevet Segal FlohMarcos Silveira BuckeridgeJanice de Almeida EnglerMaria Fatima Grossi-de-SaPublished in: Planta (2022)
The overexpression of the GmGlb1-1 gene reduces plant susceptibility to Meloidogyne incognita. Non-symbiotic globin class #1 (Glb1) genes are expressed in different plant organs, have a high affinity for oxygen, and are related to nitric oxide (NO) turnover. Previous studies showed that soybean Glb1 genes are upregulated in soybean plants under flooding conditions. Herein, the GmGlb1-1 gene was identified in soybean as being upregulated in the nematode-resistant genotype PI595099 compared to the nematode-susceptible cultivar BRS133 during plant parasitism by Meloidogyne incognita. The Arabidopsis thaliana and Nicotiana tabacum transgenic lines overexpressing the GmGlb1-1 gene showed reduced susceptibility to M. incognita. Consistently, gall morphology data indicated that pJ2 nematodes that infected the transgenic lines showed developmental alterations and delayed parasitism progress. Although no significant changes in biomass and seed yield were detected, the transgenic lines showed an elongated, etiolation-like growth under well-irrigation, and also developed more axillary roots under flooding conditions. In addition, transgenic lines showed upregulation of some important genes involved in plant defense response to oxidative stress. In agreement, higher hydrogen peroxide accumulation and reduced activity of reactive oxygen species (ROS) detoxification enzymes were also observed in these transgenic lines. Thus, based on our data and previous studies, it was hypothesized that constitutive overexpression of the GmGlb1-1 gene can interfere in the dynamics of ROS production and NO scavenging, enhancing the acquired systemic acclimation to biotic and abiotic stresses, and improving the cellular homeostasis. Therefore, these collective data suggest that ectopic or nematode-induced overexpression, or enhanced expression of the GmGlb1-1 gene using CRISPR/dCas9 offers great potential for application in commercial soybean cultivars aiming to reduce plant susceptibility to M. incognita.
Keyphrases
- genome wide
- genome wide identification
- hydrogen peroxide
- nitric oxide
- copy number
- reactive oxygen species
- transcription factor
- cell proliferation
- oxidative stress
- arabidopsis thaliana
- dna damage
- genome wide analysis
- dna methylation
- poor prognosis
- lymph node
- cell death
- squamous cell carcinoma
- cell wall
- crispr cas
- big data
- machine learning
- artificial intelligence
- deep learning
- climate change
- radiation therapy
- data analysis
- rectal cancer
- induced apoptosis
- risk assessment
- postmenopausal women
- sentinel lymph node
- endothelial cells
- binding protein