Qualitative and quantitative detection of chicken deoxyribonucleic acid (DNA) in dry dog foods.
K HołdaM Natonek-WiśniewskaP KrzyścinRobert GłogowskiPublished in: Journal of animal physiology and animal nutrition (2018)
Chicken is a common protein source in pet foods and is concurrently listed among food allergens. Commercial over-the-counter (OTC) diets with an alternative animal protein source are considered suitable for dietary elimination trials by pet owners. The potential presence of undeclared chicken-derived ingredients in these diets can compromise the outcome of the trial during the diagnosis of adverse food reactions. The aim of this study was to selectively verify the absence or presence of chicken DNA in 10 OTC dry canine foods, using qualitative and quantitative approaches. The method of identification of chicken-derived protein was elaborated with the polymerase chain reaction (PCR) technology, whereas quantitative real-time PCR was used for the quantitative assessment. In most of the analysed samples, the chicken DNA was detectable; however, the quantified amounts were predominantly low, although differences between batches were observed.
Keyphrases
- real time pcr
- circulating tumor
- high resolution
- single molecule
- cell free
- protein protein
- computed tomography
- systematic review
- positron emission tomography
- weight loss
- pet ct
- human health
- emergency department
- randomized controlled trial
- binding protein
- nucleic acid
- risk assessment
- mass spectrometry
- climate change
- african american
- pet imaging
- phase iii
- sensitive detection
- phase ii
- drug induced