Perturbation-Specific Transcriptional Mapping for unbiased target elucidation of antibiotics.

Keith P Romano Josephine Bagnall Thulasi Warrier Jaryd R Sullivan Kristina Ferrara Marek Orzechowski Phuong H Nguyen Kyra Raines Jonathan Livny Noam Shoresh Deborah T Hung

Published in: bioRxiv : the preprint server for biology (2024)

based on correlations between the expression profiles of new molecules and known perturbations, either small molecule inhibition by known antibiotics or transcriptional repression of essential targets by CRISPRi. By rapidly assigning MOAs to three new molecules with anti-pseudomonal activity, we provide proof-of-concept for a rapid, comprehensive, systematic, reference-based approach to MOA prediction with the potential to transform antibiotic discovery efforts.

Keyphrases

small molecule
gene expression
transcription factor
protein protein
high resolution
heat shock
quality improvement
high throughput
high density
human health
mass spectrometry
risk assessment
single cell
heat shock protein