Lipocalin-2 (LCN2), an effector molecule of the innate immune system that is small enough to be tagged as a reporter molecule, can be coupled with the ferric ion through a siderophore such as enterobactin (Ent). Mintbody (modification-specific intracellular antibody) can track a posttranslational protein modification in epigenetics. We constructed plasmids expressing the LCN2 hybrid of mintbody to examine the potential of LCN2 as a novel reporter for magnetic resonance imaging (MRI). Cells expressing the LCN2 hybrid of mintbody showed proper expression and localization of the hybrid and responded reasonably to Ent, suggesting their potential for in vivo study by MRI.
Keyphrases
- magnetic resonance imaging
- induced apoptosis
- contrast enhanced
- cell cycle arrest
- crispr cas
- computed tomography
- poor prognosis
- escherichia coli
- diffusion weighted imaging
- endoplasmic reticulum stress
- human health
- signaling pathway
- magnetic resonance
- oxidative stress
- binding protein
- risk assessment
- regulatory t cells
- pi k akt
- long non coding rna
- klebsiella pneumoniae
- climate change