Sfp1 regulates transcriptional networks driving cell growth and division through multiple promoter-binding modes.
Benjamin AlbertSusanna TomassettiYvonne GloorDaniel DilgStefano MattarocciSlawomir KubikLukas HafnerDavid ShorePublished in: Genes & development (2019)
The yeast Sfp1 protein regulates both cell division and growth but how it coordinates these processes is poorly understood. We demonstrate that Sfp1 directly controls genes required for ribosome production and many other growth-promoting processes. Remarkably, the complete set of Sfp1 target genes is revealed only by a combination of ChIP (chromatin immunoprecipitation) and ChEC (chromatin endogenous cleavage) methods, which uncover two promoter binding modes, one requiring a cofactor and the other a DNA-recognition motif. Glucose-regulated Sfp1 binding at cell cycle "START" genes suggests that Sfp1 controls cell size by coordinating expression of genes implicated in mass accumulation and cell division.
Keyphrases
- genome wide
- transcription factor
- cell cycle
- single cell
- gene expression
- genome wide identification
- dna binding
- cell therapy
- dna damage
- bioinformatics analysis
- high throughput
- poor prognosis
- stem cells
- genome wide analysis
- blood pressure
- oxidative stress
- adipose tissue
- metabolic syndrome
- skeletal muscle
- small molecule
- weight loss
- bone marrow