Next-generation Serology by Mass Spectrometry: Readout of the SARS-CoV-2 Antibody Repertoire.
Rafael Donadélli MelaniBenjamin J Des SoyeJared O KafaderEleonora ForteMichael HollasVoislav BlagojevicFernanda NegrãoJohn P McGeeBryon DrownCameron Lloyd-JonesHenrique S SecklerJeannie M CamarilloPhilip D ComptonRichard D LeDucBryan EarlyRyan T FellersByoung-Kyu ChoBasil Baby MattamanaYoung Ah GooPaul M ThomasMichelle K AshPavan P BhimalliLena Al-HarthiBeverly E ShaJeffrey R SchneiderMichael P SnyderPublished in: medRxiv : the preprint server for health sciences (2021)
Methods of antibody detection are used to assess exposure or immunity to a pathogen. Here, we present Ig-MS , a novel serological readout that captures the immunoglobulin (Ig) repertoire at molecular resolution, including entire variable regions in Ig light and heavy chains. Ig-MS uses recent advances in protein mass spectrometry (MS) for multi-parametric readout of antibodies, with new metrics like Ion Titer (IT) and Degree of Clonality (DoC) capturing the heterogeneity and relative abundance of individual clones without sequencing of B cells. We apply Ig-MS to plasma from subjects with severe & mild COVID-19, using the receptor-binding domain (RBD) of the spike protein of SARS-CoV-2 as the bait for antibody capture. Importantly, we report a new data type for human serology, with compatibility to any recombinant antigen to gauge our immune responses to vaccination, pathogens, or autoimmune disorders.
Keyphrases
- mass spectrometry
- sars cov
- liquid chromatography
- multiple sclerosis
- capillary electrophoresis
- gas chromatography
- respiratory syndrome coronavirus
- high performance liquid chromatography
- immune response
- ms ms
- high resolution
- coronavirus disease
- endothelial cells
- single cell
- amino acid
- protein protein
- single molecule
- drug induced
- small molecule
- electronic health record
- big data
- antimicrobial resistance
- cell free
- ultrasound guided