Electrochemiluminescence platform for transcription factor diagnosis by using CRISPR-Cas12a trans-cleavage activity.
Zhenqiang FanYuedi DingBo YaoJiaying WangKai ZhangPublished in: Chemical communications (Cambridge, England) (2021)
Herein, we exploited the double-stranded DNA (dsDNA) binding property of transcription factor (TF), combined with the trans cleavage characteristic of CRISPR-Cas12a, for the detection of NF-κB p50.
Keyphrases
- crispr cas
- dna binding
- transcription factor
- genome editing
- signaling pathway
- circulating tumor
- lps induced
- binding protein
- single molecule
- nucleic acid
- cell free
- sensitive detection
- loop mediated isothermal amplification
- high throughput
- oxidative stress
- nuclear factor
- genome wide identification
- pi k akt
- label free
- immune response