Differentially expressed genes of esophageal tissue in male acute and chronic sleep deprivation mice.
Jing LiYifan LuDingding YangMudan RenYan YinYan ZhaoShui-Xiang HePublished in: Genes, brain, and behavior (2024)
Gastroesophageal reflux disease (GERD) is associated with sleep disturbances. However, mechanisms underlying these interactions remain unclear. Male acute and chronic sleep deprivation (SD) mice were used for this study. Mice in the chronic SD group exhibited anxiety- and depression-like behaviors. We further performed high-throughput genome sequencing and bioinformatics analysis to screen for featured differentially expressed genes (DEGs) in the esophageal tissue. The acute SD group, comprised 25 DEGs including 14 downregulated and 11 upregulated genes. Compared with the acute SD group, more DEGs were present in the chronic SD group, with a total of 169 DEGs, including 88 downregulated and 81 upregulated genes. Some DEGs that were closely related to GERD and associated esophageal diseases were significantly different in the chronic SD group. Quantitative real-time polymerase chain reaction verified the downregulation of Krt4, Krt13, Krt15 and Calml3 and upregulation of Baxl1 and Per3. Notably, these DEGs are involved in biological processes, which might be the pathways of the neuroregulatory mechanisms of DEGs expression.
Keyphrases
- bioinformatics analysis
- liver failure
- drug induced
- high throughput
- respiratory failure
- genome wide
- gastroesophageal reflux disease
- aortic dissection
- poor prognosis
- physical activity
- cell proliferation
- high fat diet induced
- signaling pathway
- single cell
- type diabetes
- gene expression
- long non coding rna
- skeletal muscle
- binding protein
- acute respiratory distress syndrome
- mechanical ventilation