RDP3, A Novel Antigout Peptide Derived from Water Extract of Rice.
Naixin LiuYing WangLin ZengSaige YinYan HuShanshan LiYang FuXinping ZhangChun XieLongjun ShuYilin LiHuiling SunMeifeng YangJun SunMeifeng YangPublished in: Journal of agricultural and food chemistry (2020)
Gout and hyperuricemia can seriously affect the quality of life; at present, however, existing medicines are unable to meet all clinical needs. In the current study, a novel peptide (i.e., rice-derived-peptide-3 (RDP3), AAAAMAGPK-NH2, 785.97 Da) in water extract obtained from shelled Oryza sativa fruits was identified. Testing revealed that RDP3 (minimum effective concentration 100 μg/kg) did not show both hemolytic and acute toxicity, and reduced uric acid levels in the serum of hyperuricemic mice by inhibiting xanthine oxidase activity and decreasing urate transporter 1 expression. RDP3 also alleviated renal injury in hyperuricemic mice by decreasing NLRP3 inflammasome expression. Furthermore, RDP3 alleviated formalin-induced paw pain and reduced monosodium urate crystal-induced paw swelling and inflammatory factors in mice. Thus, this newly identified peptide reduced uric acid levels and renal damage in hyperuricemic mice and showed anti-inflammatory and analgesic activities, indicating the potential of RDP3 as an antigout medicine candidate.
Keyphrases
- uric acid
- metabolic syndrome
- anti inflammatory
- oxidative stress
- high fat diet induced
- nlrp inflammasome
- poor prognosis
- diabetic rats
- high glucose
- drug induced
- chronic pain
- neuropathic pain
- spinal cord injury
- insulin resistance
- liver failure
- type diabetes
- adipose tissue
- long non coding rna
- single cell
- intensive care unit
- spinal cord
- skeletal muscle
- risk assessment
- climate change
- room temperature
- human health