Inhibitory activity of hinokitiol against biofilm formation in fluconazole-resistant Candida species.
Dae Jin KimMin Woo LeeJeong Su ChoiSeung Gwan LeeJee Yoon ParkSuhng Wook KimPublished in: PloS one (2017)
The aim of this study was to investigate the ability of hinokitiol to inhibit the formation of Candida biofilms. Biofilm inhibition was evaluated by quantification of the biofilm metabolic activity with XTT assay. Hinokitiol efficiently prevented biofilm formation in both fluconazole-susceptible and fluconazole-resistant strains of Candida species. We determined the expression levels of specific genes previously implicated in biofilm development of C. albicans cells by real-time RT-PCR. The expression levels of genes associated with adhesion process, HWP1 and ALS3, were downregulated by hinokitiol. Transcript levels of UME6 and HGC1, responsible for long-term hyphal maintenance, were also decreased by hinokitiol. The expression level of CYR1, which encodes the component of signaling pathway of hyphal formation-cAMP-PKA was suppressed by hinokitiol. Its upstream general regulator RAS1 was also suppressed by hinokitiol. These results indicate that hinokitiol may have therapeutic potential in the treatment and prevention of biofilm-associated Candida infections.
Keyphrases
- candida albicans
- biofilm formation
- poor prognosis
- signaling pathway
- binding protein
- induced apoptosis
- pseudomonas aeruginosa
- escherichia coli
- staphylococcus aureus
- gene expression
- transcription factor
- high throughput
- genome wide
- cell death
- long non coding rna
- endoplasmic reticulum stress
- oxidative stress
- protein kinase
- cystic fibrosis
- cell migration
- real time pcr