DEAD-box RNA helicase protein DDX21 as a prognosis marker for early stage colorectal cancer with microsatellite instability.
Atsushi TanakaJulia Y WangJinru ShiaYihua ZhouMakiko OgawaRonald C HendricksonDavid S KlimstraMichael H A RoehrlPublished in: Scientific reports (2020)
DEAD-box RNA helicase DDX21 (also named nucleolar RNA helicase 2) is a nuclear autoantigen with undefined roles in cancer. To explore possible roles of autoimmune recognition in cancer immunity, we examined DDX21 protein expression in colorectal cancer tissue and its association with patient clinical outcomes. Unbiased deep proteomic profiling of two independent colorectal cancer cohorts using mass spectrometry showed that DDX21 protein was significantly upregulated in cancer relative to benign mucosa. We then examined DDX21 protein expression in a validation group of 710 patients, 619 of whom with early stage and 91 with late stage colorectal cancers. DDX21 was detected mostly in the tumor cell nuclei, with high expression in some mitotic cells. High levels of DDX21 protein were found in 28% of stage I, 21% of stage II, 30% of stage III, and 32% of stage IV colorectal cancer cases. DDX21 expression levels correlated with non-mucinous histology in early stage cancers but not with other clinicopathological features such as patient gender, age, tumor location, tumor grade, or mismatch repair status in any cancer stage. Kaplan-Meier analyses revealed that high DDX21 protein levels was associated with longer survival in patients with early stage colorectal cancer, especially longer disease-free survival in patients with microsatellite instability (MSI) cancers, but no such correlations were found for the microsatellite stable subtype or late stage colorectal cancer. Univariate and multivariate analyses also identified high DDX21 protein expression as an independent favorable prognostic marker for early stage MSI colorectal cancer.
Keyphrases
- early stage
- papillary thyroid
- binding protein
- squamous cell
- mass spectrometry
- poor prognosis
- protein protein
- case report
- childhood cancer
- lymph node metastasis
- single cell
- transcription factor
- mental health
- squamous cell carcinoma
- chronic kidney disease
- multiple sclerosis
- amino acid
- young adults
- high resolution
- bone marrow
- radiation therapy
- stem cells
- signaling pathway
- ejection fraction
- cell cycle
- peritoneal dialysis
- mesenchymal stem cells
- data analysis
- genome wide analysis