A native mass spectrometry approach to qualitatively elucidate interfacial epitopes of transient protein-protein interactions.
Clinton G L VealeAbir ChakrabortyRichwell MhlangaFernando AlbericioBeatriz G de la TorreAdrienne L EdkinsDavid James ClarkPublished in: Chemical communications (Cambridge, England) (2024)
Native mass spectrometric analysis of TPR2A and GrpE with unpurified peptides derived from limited proteolysis of their respective PPI partners (HSP90 C-terminus and DnaK) facilitated efficient, qualitative identification of interfacial epitopes involved in transient PPI formation. Application of this approach can assist in elucidating interfaces of currently uncharacterised transient PPIs.