Cell reprogramming using extracellular vesicles from differentiating stem cells into white/beige adipocytes.
Youn Jae JungHark Kyun KimYoonsuk ChoJi Suk ChoiChang Hee WooKyoung Soo LeeJae Hoon SulChan Mi LeeJihoon HanJae Hyung ParkDong-Gyu JoYong Woo ChoPublished in: Science advances (2020)
Stem cell-derived extracellular vesicles (EVs) offer alternative approaches to stem cell-based therapy for regenerative medicine. In this study, stem cell EVs derived during differentiation are developed to use as cell-free therapeutic systems by inducing tissue-specific differentiation. EVs are isolated from human adipose-derived stem cells (HASCs) during white and beige adipogenic differentiation (D-EV and BD-EV, respectively) via tangential flow filtration. D-EV and BD-EV can successfully differentiate HASCs into white and beige adipocytes, respectively. D-EV are transplanted with collagen/methylcellulose hydrogels on the backs of BALB/c mice, and they produce numerous lipid droplets in injected sites. Treatments of BD-EV attenuate diet-induced obesity through browning of adipose tissue in mice. Furthermore, high-fat diet-induced hepatic steatosis and glucose tolerance are improved by BD-EV treatment. miRNAs are responsible for the observed effects of BD-EV. These results reveal that secreted EVs during stem cell differentiation into white adipocytes or beige adipocytes can promote cell reprogramming.
Keyphrases
- high fat diet induced
- stem cells
- insulin resistance
- adipose tissue
- cell therapy
- cell free
- single cell
- high fat diet
- metabolic syndrome
- skeletal muscle
- type diabetes
- weight loss
- magnetic resonance imaging
- fatty acid
- magnetic resonance
- gene expression
- genome wide
- wound healing
- tissue engineering
- drug release
- induced pluripotent stem cells