Electrochemical detection of glutathione based on accelerated CRISPR/Cas12a trans -cleavage with MnO 2 nanosheets.
Renpeng XiaNan OuyangTingting WangYuan ZhuangPeng MiaoPublished in: Chemical communications (Cambridge, England) (2024)
The CRISPR/Cas12a system is accelerated by glutathione-mediated reduction of MnO 2 nanosheets. By monitoring the trans -cleavage of the DNA probe, an electrochemical method for glutathione assay is fabricated, with the detection limit of 3.5 pM. It provides a promising tool for plasma analysis with satisfactory performance, indicating the broad application prospects of this glutathione assay.
Keyphrases
- crispr cas
- label free
- genome editing
- gold nanoparticles
- quantum dots
- reduced graphene oxide
- loop mediated isothermal amplification
- ionic liquid
- real time pcr
- dna binding
- air pollution
- single molecule
- molecularly imprinted
- particulate matter
- highly efficient
- circulating tumor
- metal organic framework
- cell free
- living cells
- heavy metals
- current status
- polycyclic aromatic hydrocarbons
- transcription factor
- liquid chromatography