Liquid chromatography/mass spectrometry based detection and semi-quantitative analysis of INSL5 in human and murine tissues.

Richard G Kay S Galvin Pierre Larraufie Frank Reimann Fiona M Gribble

Published in: Rapid communications in mass spectrometry : RCM (2018)

The first LC/MS/MS-based method capable of the detection and semi-quantitative analysis of endogenous INSL5 using MS-based techniques has been demonstrated. The methodology will enable the identification of stimulants for INSL5 secretion from murine and human primary colonic epithelial cultures.

Keyphrases

mass spectrometry
liquid chromatography
endothelial cells
induced pluripotent stem cells
high resolution
pluripotent stem cells
loop mediated isothermal amplification
high resolution mass spectrometry
multiple sclerosis
gene expression
real time pcr
label free
simultaneous determination
sensitive detection