Umbilical Cord-Derived Mesenchymal Stem Cells Relieve Hindlimb Ischemia through Enhancing Angiogenesis in Tree Shrews.
Cunping YinYuan LiangJian ZhangGuang-Ping RuanZian LiRongqing PangXinghua PanPublished in: Stem cells international (2016)
Hindlimb ischemia is still a clinical problem with high morbidity and mortality. Patients suffer from consequent rest pain, ulcers, cool limbs, and even amputation. Angiogenesis is a promising target for the treatment of ischemic limbs, providing extra blood for the ischemic region. In the present study, we investigated the role of umbilical cord-derived mesenchymal stem cells (UC-MSCs) in regulating angiogenesis and relieving hindlimb ischemia. UC-MSCs were isolated from the umbilical cord of tree shrews. Angiography results showed that UC-MSCs injection significantly promoted angiogenesis in tree shrews. Moreover, the ankle brachial index, transcutaneous oxygen pressure, blood perfusion, and capillary/muscle fiber ratio were all markedly increased by the application of UC-MSCs. In addition, the conditioned culture of human umbilical vein endothelial cells using medium collected from UC-MSCs showed higher expression of angiogenic markers and improved migration ability. In short, the isolated UC-MSCs notably contributed to restoring blood supply and alleviating the symptoms of limb ischemia through enhancing angiogenesis.
Keyphrases
- umbilical cord
- mesenchymal stem cells
- endothelial cells
- vascular endothelial growth factor
- wound healing
- end stage renal disease
- high glucose
- bone marrow
- chronic kidney disease
- chronic pain
- poor prognosis
- optical coherence tomography
- ejection fraction
- newly diagnosed
- computed tomography
- peritoneal dialysis
- pain management
- neuropathic pain
- cerebral ischemia
- long non coding rna
- high speed
- replacement therapy
- patient reported
- peripheral artery disease