Rapid generation and selection of Cas9-engineering TRP53 R172P mice that do not have off-target effects.

Guoxing ZhengQingqing ZhuJunchao DongXin LinChengming Zhu

Published in: BMC biotechnology (2019)

A single injection of the this optimized CRISPR/Cas9 system can be applied to introduce particular mutations in the genome of mice without off-target effects to model various human diseases.

Keyphrases

crispr cas
genome editing
endothelial cells
high fat diet induced
type diabetes
gene expression
dna methylation
induced pluripotent stem cells
adipose tissue
quantum dots