ZnT3 expression levels are down-regulated in the brain of Mcoln1 knockout mice.

RNA-seq analysis showed a marked decrease in baseline levels of Slc30a3 mRNA in Mcoln1-/- mice. Real-time qPCR and Western blot analyses confirmed that Slc30a3 transcripts and its protein levels were significantly reduced. Our observations add MLIV to a growing list of neurodegenerative diseases that parallels abnormal ZnT3 expression with zinc dyshomeostasis.