CRISPR-Cas-Induced Mutants Identify a Requirement for dSTIM in Larval Dopaminergic Cells of Drosophila melanogaster.
Trayambak PathakDeepti TrivediGaiti HasanPublished in: G3 (Bethesda, Md.) (2017)
Molecular components of store-operated calcium entry have been identified in the recent past and consist of the endoplasmic reticulum (ER) membrane-resident calcium sensor STIM and the plasma membrane-localized calcium channel Orai. The physiological function of STIM and Orai is best defined in vertebrate immune cells. However, genetic studies with RNAi strains in Drosophila suggest a role in neuronal development and function. We generated a CRISPR-Cas-mediated deletion for the gene encoding STIM in Drosophila (dSTIM), which we demonstrate is larval lethal. To study STIM function in neurons, we merged the CRISPR-Cas9 method with the UAS-GAL4 system to generate either tissue- or cell type-specific inducible STIM knockouts (KOs). Our data identify an essential role for STIM in larval dopaminergic cells. The molecular basis for this cell-specific requirement needs further investigation.
Keyphrases
- crispr cas
- drosophila melanogaster
- genome editing
- endoplasmic reticulum
- induced apoptosis
- cell cycle arrest
- genome wide
- aedes aegypti
- spinal cord
- endoplasmic reticulum stress
- signaling pathway
- patient safety
- copy number
- cell death
- oxidative stress
- single molecule
- stem cells
- gene expression
- cell therapy
- diabetic rats
- machine learning
- brain injury
- pi k akt
- spinal cord injury