Actinoplanes kirromycinicus sp. nov., isolated from soil.

A novel actinomycete, designated as TPMA0078 T , was isolated from a soil sample collected in Shinjuku, Tokyo, Japan. 16S rRNA gene sequence analysis indicated that strain TPMA0078 T belongs to the genus Actinoplanes and is closely related to Actinoplanes regularis IFO 12514 T (99.86% 16S rRNA gene sequence similarity). The spores of strain TPMA0078 T were motile, and the sporangia were cylindrical. The diamino acids in the cell wall peptidoglycan of strain TPMA0078 T were meso-diaminopimelic acid and 3OH-meso-diaminopimelic acid. Whole-cell sugars were glucose and mannose, with galactose as a minor component. The major cellular fatty acids identified were iso-C 15:0 , iso-C 16:0 , and anteiso-C 17:0 . The predominant menaquinone was MK-9(H 4 ), and the principal polar lipid was phosphatidylethanolamine. These chemotaxonomic properties of strain TPMA0078 T were consistent with those of Actinoplanes. Meanwhile, digital DNA-DNA hybridization and average nucleotide identity values showed low relatedness between strain TPMA0078 T and A. regularis NBRC 12514 T . Furthermore, several phenotypic properties of strain TPMA0078 T distinguished it from those of closely related species. Based on its genotypic and phenotypic characteristics, strain TPMA0078 T represents a novel species of the genus Actinoplanes, for which the name Actinoplanes kirromycinicus sp. nov. is proposed. The type strain is TPMA0078 T (=NBRC 116422 T  = TBRC 18262 T ).