Development of a counterselectable seamless mutagenesis system in lactic acid bacteria.
Yongping XinTingting GuoYingli MuJian KongPublished in: Microbial cell factories (2017)
As reported in E. coli, pheS* as a counterselectable marker has been demonstrated to be functional in targeted gene(s) deletion in Lc. lactis as well as in L. casei. Moreover, the efficiency and timesaving counterselectable seamless mutagenesis system PheS*/pG+host9 could be used in the wild-type host cells without pretreatment.
Keyphrases
- lactic acid
- wild type
- crispr cas
- induced apoptosis
- cell cycle arrest
- escherichia coli
- genome wide
- copy number
- cancer therapy
- simultaneous determination
- mass spectrometry
- cell death
- oxidative stress
- signaling pathway
- genome wide identification
- transcription factor
- high resolution
- pi k akt
- tandem mass spectrometry
- high resolution mass spectrometry