Allicin Could Potentially Alleviate Oral Cancer Pain by Inhibiting "Pain Mediators" TNF-alpha, IL-8, and Endothelin.
Abdulwahab H AlamirShankaragouda PatilPublished in: Current issues in molecular biology (2021)
To evaluate the effects of allicin on mediators of pain secreted by oral cancer cells in vitro, single-cell suspensions were prepared by enzymatic method from oral squamous cell carcinoma (OSCC). Cancer stem cells were isolated by the CD133+ selection method with magnetic cell sorting. Stemness markers were checked in both cancer cells and cancer stem cells by RT-PCR. Comparative analysis of pain mediators TNF-alpha, IL-8, and endothelin at both RNA and protein levels for normal epithelial cells, cancer cells, and cancer stem cells was carried out with and without allicin treatment. CD133 and CD44 expression levels were checked in cancer cells and cancer stem cells flow cytometrically. Allicin inhibited both gene and protein expression of TNF-alpha, IL-8, and endothelin in both cancer cells and cancer stem cells. Allicin is more likely to be a promising treatment in alleviating the levels of pain and inflammation in OSCCs.
Keyphrases
- cancer stem cells
- chronic pain
- pain management
- neuropathic pain
- single cell
- rheumatoid arthritis
- oxidative stress
- poor prognosis
- signaling pathway
- spinal cord
- rna seq
- gene expression
- copy number
- small molecule
- hydrogen peroxide
- binding protein
- mass spectrometry
- mesenchymal stem cells
- amino acid
- protein protein
- solid phase extraction
- simultaneous determination