Cytogenotoxic screening of the natural compound niga-ichigoside F1 from Rubus imperialis (Rosaceae).
Laíza Moura Almeida-TerassiAna Paula Martins CastanhaGreice Rafaele AlvesValdir Cechinel-FilhoRivaldo NieroIsabel O'Neill de Mascarenhas GaivãoLiana Martins de OliveiraLuan Vitor Alves de LimaMario Sergio MantovaniEdson Luis MaistroPublished in: Journal of applied toxicology : JAT (2024)
Rubus imperialis Chum. Schl. (Rosaceae) have demonstrated some pharmacological activities, including gastroprotective action. However, genotoxic effects of R. imperialis extract was also reported. Since niga-ichigoside F1 (NIF1) is a major compound of this plant species, and which has proven pharmacological properties, it is essential to investigate whether this compound is responsible for the observed toxicity. Therefore, the objective of this study was to analyze the effects of NIF1 on HepG2/C3A cells for possible cytogenotoxicity, cell cycle and apoptosis influence, and expression of genes linked to the DNA damage, cell cycle, cell death, and xenobiotic metabolism. The results showed no cytogenotoxic effects of NIF1 at concentrations between 0.1 and 20 μg/ml. Flow cytometry also showed no cell cycle or apoptosis disturbance. In the gene expression analysis, none of the seven genes investigated showed altered expression. The data indicate that NIF1 has no cytogenotoxic effects, and no interruption of the cell cycle, or induction of apoptosis, apparently not being responsible for the cytotoxic effects observed in the crude extract of R. imperialis.
Keyphrases
- cell cycle
- cell cycle arrest
- cell death
- oxidative stress
- cell proliferation
- dna damage
- induced apoptosis
- pi k akt
- genome wide identification
- flow cytometry
- endoplasmic reticulum stress
- poor prognosis
- genome wide
- signaling pathway
- long non coding rna
- transcription factor
- anti inflammatory
- binding protein
- big data
- gene expression
- dna repair
- bioinformatics analysis