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Comparative Evaluation on Vitamin E and Carotenoid Accumulation in Sweet Corn (Zea mays L.) Seedlings under Temperature Stress.

Nan XiangChunyan LiGaoke LiYongtao YuJianguang HuXin-Bo Guo
Published in: Journal of agricultural and food chemistry (2019)
This study aims to investigate the response profiles of vitamin E and carotenoids on transcription and metabolic levels of sweet corn seedlings under temperature stress. The treated temperatures were set as 10 °C (low temperature, LT), 25 °C (control, CK), and 40 °C (high temperature, HT) for sweet corn seedlings. The gene expression profiles of vitamin E and carotenoids biosynthesis pathways were analyzed by real time quantitative polymerase chain reaction (RT-qPCR), and the composition profiles were analyzed by high performance liquid chromatography (HPLC). Results showed that vitamin E gradually accumulated in response to LT stress but was limited by HT stress. The increase of carotenoids was suppressed by LT stress whereas HT stress promoted it. The existing results elaborated the interactive and competitive relationships of vitamin E and carotenoids in sweet corn seedlings to respond to extreme temperature stress at transcriptional and metabolic levels. The present study would improve sweet corn temperature resilience with integrative knowledge in the future.
Keyphrases
  • high performance liquid chromatography
  • healthcare
  • mass spectrometry
  • gene expression
  • simultaneous determination
  • heat stress
  • genome wide
  • high temperature
  • solid phase extraction
  • newly diagnosed