DNAzyme-activated CRISPR/Cas assay for sensitive and one-pot detection of lead contamination.
Ruijie DengYaxuan BaiYumei LiuYunhao LuZhifeng ZhaoYi DengHao YangPublished in: Chemical communications (Cambridge, England) (2024)
Hazardous lead ions (Pb 2+ ) even at a minute level can pose side effects on human health, highlighting the need for tools for trace Pb 2+ detection. Herein, we present a DNAzyme-activated CRISPR assay (termed DzCas12T) for sensitive and one-pot detection of lead contamination. Using an extension-bridged strategy eliminates the need for separation to couple the DNAzyme recognition and CRISPR reporting processes. The tandem design endowed the DzCas12T assay with high specificity and sensitivity down to the pM-level. This assay has been used to detect lead contamination in food and water samples, indicating the potential for monitoring lead-associated environmental and food safety.
Keyphrases
- human health
- risk assessment
- crispr cas
- heavy metals
- label free
- genome editing
- high throughput
- climate change
- loop mediated isothermal amplification
- living cells
- real time pcr
- health risk
- drinking water
- air pollution
- gene expression
- emergency department
- fluorescent probe
- particulate matter
- mass spectrometry
- sensitive detection