The epigenetic control of stemness in CD8+ T cell fate commitment.
Luigia PaceChristel GoudotElina ZuevaPaul GueguenNina BurgdorfJoshua J WaterfallJean-Pierre QuivyGeneviève AlmouzniSebastian AmigorenaPublished in: Science (New York, N.Y.) (2018)
After priming, naïve CD8+ T lymphocytes establish specific heritable transcription programs that define progression to long-lasting memory cells or to short-lived effector cells. Although lineage specification is critical for protection, it remains unclear how chromatin dynamics contributes to the control of gene expression programs. We explored the role of gene silencing by the histone methyltransferase Suv39h1. In murine CD8+ T cells activated after Listeria monocytogenes infection, Suv39h1-dependent trimethylation of histone H3 lysine 9 controls the expression of a set of stem cell-related memory genes. Single-cell RNA sequencing revealed a defect in silencing of stem/memory genes selectively in Suv39h1-defective T cell effectors. As a result, Suv39h1-defective CD8+ T cells show sustained survival and increased long-term memory reprogramming capacity. Thus, Suv39h1 plays a critical role in marking chromatin to silence stem/memory genes during CD8+ T effector terminal differentiation.
Keyphrases
- gene expression
- single cell
- genome wide
- working memory
- stem cells
- dna methylation
- induced apoptosis
- transcription factor
- rna seq
- cell cycle arrest
- public health
- dna damage
- listeria monocytogenes
- dendritic cells
- regulatory t cells
- poor prognosis
- high throughput
- bioinformatics analysis
- cell proliferation
- oxidative stress
- cell death
- signaling pathway
- cell fate
- amino acid
- drug induced
- free survival