The role and mechanism of unfolded protein response signaling pathway in methylmercury-induced apoptosis of mouse spermatocytes germ cell-2 cells.
Xiayu ZhangHuifang HaoKai MaHuan PangXinyue LiTiantian TianShanshan HouXiaofan NingHao WuQiaohong HouMeng LiYunxiang SunXiuling SongMinghua JinPublished in: Environmental toxicology (2022)
The study aimed to explore the role and mechanism of unfolded protein response (UPR) in methylmercury (MeHg)-induced Mouse Spermatocytes (GC-2spd[ts]) apoptosis. Methods such as MTT, flow cytometry, and Western Blot were used to evaluate the cell viability, membrane potential (MMP), reactive oxygen species (ROS), calcium ion (Ca 2+ ), rate of cell apoptosis, and the expression of apoptosis-related and UPR-related protein. The results showed that with the increase of MeHg concentration, cell viability and MMP decreased, ROS, Ca 2+ , rate of cell apoptosis, and the expression of apoptosis-related protein and UPR-related protein increased. To further explore the effect of ROS-induced oxidative damage on it, the ROS inhibitor N-acetyl-L-cysteine (NAC) was used. The effects of MeHg on germ cell (GC-2) cells were partially inhibited after NAC pretreatment. Our present study proved that MeHg might induce cell apoptosis by activating the UPR signaling pathway in GC-2 cells and affect normal reproductive function.
Keyphrases
- induced apoptosis
- endoplasmic reticulum stress
- signaling pathway
- cell cycle arrest
- cell death
- oxidative stress
- reactive oxygen species
- pi k akt
- germ cell
- dna damage
- diabetic rats
- poor prognosis
- flow cytometry
- cell proliferation
- transcription factor
- binding protein
- high glucose
- epithelial mesenchymal transition
- risk assessment
- drug induced
- south africa