Pre-clinical study of induced pluripotent stem cell-derived dopaminergic progenitor cells for Parkinson's disease.
Daisuke DoiHiroaki MagotaniTetsuhiro KikuchiMegumi IkedaSatoe HiramatsuKenji YoshidaNaoki AmanoMasaki NomuraMasafumi UmekageAsuka MorizaneJun TakahashiPublished in: Nature communications (2020)
Induced pluripotent stem cell (iPSC)-derived dopaminergic (DA) neurons are an expected source for cell-based therapies for Parkinson's disease (PD). The regulatory criteria for the clinical application of these therapies, however, have not been established. Here we show the results of our pre-clinical study, in which we evaluate the safety and efficacy of dopaminergic progenitors (DAPs) derived from a clinical-grade human iPSC line. We confirm the characteristics of DAPs by in vitro analyses. We also verify that the DAP population include no residual undifferentiated iPSCs or early neural stem cells and have no genetic aberration in cancer-related genes. Furthermore, in vivo studies using immunodeficient mice reveal no tumorigenicity or toxicity of the cells. When the DAPs are transplanted into the striatum of 6-OHDA-lesioned rats, the animals show behavioral improvement. Based on these results, we started a clinical trial to treat PD patients in 2018.
Keyphrases
- induced pluripotent stem cells
- stem cells
- clinical trial
- high glucose
- endothelial cells
- neural stem cells
- diabetic rats
- single cell
- end stage renal disease
- newly diagnosed
- induced apoptosis
- genome wide
- double blind
- ejection fraction
- papillary thyroid
- oxidative stress
- cell therapy
- drug induced
- prognostic factors
- transcription factor
- spinal cord
- squamous cell carcinoma
- mesenchymal stem cells
- spinal cord injury
- young adults
- squamous cell
- patient reported outcomes
- study protocol
- signaling pathway
- pluripotent stem cells
- phase ii