Epidermal melanocytes metabolize extracellular nucleotides by purinergic enzymes.
Liliana Ivet Sous NaasaniJéssica Gonçalves AzevedoJean SévignyTiago Franco de OliveiraSilvya Stuchi Maria-EnglerMarcia Rosângela WinkPublished in: Biochemistry and cell biology = Biochimie et biologie cellulaire (2023)
The human epidermal melanocyte (hEM) are melanin-producing cells that provide skin pigmentation and protection against ultraviolet radiation. Although purinergic signaling is involved in skin biology and pathology, the presence of NTPDase members, as well as the rate of nucleotides degradation by melanocytes were not described yet. Therefore, in this study, we analyzed the expression of ectonucleotidases in hEM derived from discarded foreskin of male patients. The expression of purinergic enzymes was confirmed by mRNA and flow cytometry. Among the ectonucleotidases, ectonucleoside triphosphate diphosphohydrolase1 (NTPDase1) and ecto-5´-nucleotidase were the ectoenzymes with higher expressions. The hydrolysis rate for ATP, ADP, and AMP was low in comparison to other primary cells already investigated. The amount of ATP in the culture medium was increased after a scratch wound and decreased to basal levels in 48 h, while the NTPDase1 and P2X7 expressions increased. Therefore, it is possible to suggest that after cell injury, the ATP released by hEM into the extracellular space will be hydrolyzed by ectonucleotidases as the NTPDase1 that will control the levels of nucleotides in the skin micro-environment.
Keyphrases
- wound healing
- induced apoptosis
- flow cytometry
- poor prognosis
- cell cycle arrest
- end stage renal disease
- soft tissue
- binding protein
- ejection fraction
- chronic kidney disease
- endothelial cells
- newly diagnosed
- endoplasmic reticulum stress
- single cell
- peritoneal dialysis
- prognostic factors
- cell therapy
- cell proliferation
- cell death
- radiation induced
- patient reported outcomes
- protein kinase
- clinical evaluation