PU.1 negatively regulates tumorigenesis in non-small-cell lung cancer.
Xiaoxiao LiuMuli XuWanting JiaYu DuanJiaxuan MaWenlin TaiPublished in: Medical oncology (Northwood, London, England) (2023)
PU.1 is a key transcription factor that modulates hematopoietic cell differentiation and is involved in various physiological and pathological processes. PU.1 has been described to have multiple roles in a diverse range of cancers, but its contribution in non-small-cell lung cancer (NSCLC) has not been clearly elucidated. Fifty pairs of lung adenocarcinoma (LUAD) tissues and paraneoplastic tissues were collected. RT-qPCR assay was used to test PU.1 expression. Expression of PU.1 in LUAD cell lines and control cell lines was detected by RT-qPCR, and the role of PU.1 in LUAD was investigated by in vitro experiment. Levels of the major proteins in the apoptotic pathway were also detected by Western blot. The expression of PU.1 was remarkably downregulated in LUAD. Overexpression of PU.1 impaired the viability of LUAD cells as well as their metastatic function. In addition, PU.1 promoted apoptosis of LUAD cells by decreasing Bcl2 and increasing Bax/Bak1 expression. PU.1 plays an inhibitory role in LUAD, mainly promoting the apoptosis of LUAD cells.
Keyphrases
- cell cycle arrest
- induced apoptosis
- poor prognosis
- cell death
- endoplasmic reticulum stress
- transcription factor
- oxidative stress
- small cell lung cancer
- gene expression
- squamous cell carcinoma
- binding protein
- pi k akt
- cell proliferation
- bone marrow
- signaling pathway
- long non coding rna
- single cell
- mass spectrometry
- epidermal growth factor receptor
- brain metastases