Single-molecule analysis of subtelomeres and telomeres in Alternative Lengthening of Telomeres (ALT) cells.

Heba Z AbidJennifer McCaffreyKaitlin RaseleyEleanor YoungKaty LassahnDharma VarapulaHarold RiethmanMing Xiao

Published in: BMC genomics (2020)

Identifying individual subtelomeres and characterizing linked telomere (TTAGGG) n tract lengths and structural changes using our new single-molecule methodologies reveals the structural consequences of telomere damage, repair and recombination mechanisms in human ALT cells in unprecedented molecular detail and significant differences in different ALT-positive cell lines.

Keyphrases

single molecule
induced apoptosis
atomic force microscopy
living cells
cell cycle arrest
oxidative stress
endoplasmic reticulum stress
signaling pathway
cell death
dna repair
pluripotent stem cells
high speed