Virulent potential of methicillin-resistant and methicillin-susceptible Staphylococcus pseudintermedius in dogs.
Gabriela Merker BreyerBianca Fagundes SagginSilvia de CarliMaria Eduarda Rocha Jacques da SilvaMateus Matiuzzi da CostaBertram BrenigVasco Ariston de Carvalho AzevedoMarisa Ribeiro de Itapema CardosoFranciele Maboni SiqueiraPublished in: Acta tropica (2023)
Staphylococcus pseudintermedius is a zoonotic pathogen responsible for several infectious diseases in pet animals, yet its pathogenic potential is not fully understood. Thus, this study aims to unravel the virulence profile of S. pseudintermedius from canine origin. Methicillin-resistant (MRSP) and methicillin-susceptible (MSSP) strains were isolated from different infection sites and their genotypic and phenotypic features were compared to determine the clinical implications of MRSP and MSSP strains. Bacterial identification was performed using MALDI-TOF and 16S-rDNA sequencing. In addition, we used multilocus sequence typing (MLST) for strains' sequence type (ST) determination and phylogenetic relationship. The strains were screened for toxin genes, including cytotoxins (lukS, lukF), exfoliative toxin (siet), enterotoxins (sea, seb, sec, secCanine, sel, sem, and seq) and toxic shock syndrome toxin (tst-1). In vitro phenotypic analyses assessing antimicrobial susceptibility profile, biofilm formation ability, and expression of extracellular matrix components were performed. The investigated S. pseudintermedius strains belong to 17 unique ST, most of which were classified as ST71. MSSP and MRSP strains shared siet, lukS, and lukF virulence markers. Our findings showed that some MSSP strains also harbored sel, seq, and sem enterotoxin genes, suggesting a more diverse virulence profile. All MRSP strains and 77% of MSSP strains were classified as multidrug resistant (MDR). Moreover, all investigated S. pseudintermedius strains showed strong biofilm formation ability. In summary, our findings highlight the wide spread of highly virulent and drug-resistant zoonotic S. pseudintermedius strains, being a potential concern for One Health issues.
Keyphrases
- escherichia coli
- biofilm formation
- staphylococcus aureus
- multidrug resistant
- drug resistant
- pseudomonas aeruginosa
- candida albicans
- klebsiella pneumoniae
- extracellular matrix
- healthcare
- genome wide
- methicillin resistant staphylococcus aureus
- ms ms
- risk assessment
- mental health
- antimicrobial resistance
- transcription factor
- public health
- mass spectrometry
- social media
- single cell
- infectious diseases
- binding protein
- computed tomography
- positron emission tomography
- gram negative
- poor prognosis
- liquid chromatography